Quantification of fructans in cereal‐based bioethanol stillage based on a simplified analytical method

Background and Objectives Cereal-based bioethanol stillage, the main by-product of industrial production, is a potential substrate for fructans. However, quantification fructans in such complex sample matrices still challenge corresponding analytics to be overcome allow identification utilization unused fructan sources. Especially possible or rather process development requires appropriate first. Thus, this paper aims develop apply method stillage using common high-performance liquid chromatography–refractive index detector. Findings A new approach presented allowing simple determination comparably high throughput. The developed analytical performs by means fructose- glucose-equivalent after targeted acidic hydrolysis adapted respective matrix. On basis method, up 2.5% were found dry matter cereal-based stillage. Conclusion assessed source can and, thus, may contribute higher-value Significance Novelty According current knowledge, no methods have been published so far.